1IBL
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH A MESSENGER RNA FRAGMENT AND COGNATE TRANSFER RNA ANTICODON STEM-LOOP BOUND AT THE A SITE AND WITH THE ANTIBIOTIC PAROMOMYCIN
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-02-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.99187 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 400.800, 400.800, 175.885 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 316.230 - 3.110 |
| R因子 | 0.232 * |
| Rwork | 0.232 |
| R-free | 0.27500 |
| Structure solution method | DIFFERENCE FOURIER |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 28.380 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 316.230 | 3.220 |
| 分解能 [Å] (高) | 3.110 | 3.110 |
| Rmerge_l_obs | 0.117 * | 0.578 * |
| Total number of observations | 1170885 * | |
| 独立反射数 | 240896 | |
| <I/σ(I)> | 11.9 | 2.8 |
| 完全性 [%] | 95.1 | 92.7 |
| 冗長性 | 4.86 | 3.51 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | Wimberly, B.T., (2000) Nature, 407, 327.,Trakhanov, S.D., (1987) FEBS Lett., 220, 319. * |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | NH4Cl | ||
| 10 | 1 | 2 | MPD | ||
| 11 | 1 | 3 | paromomycin | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | sodium cacodylate | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | NH4Cl | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | magnesium acetate | ||
| 9 | 1 | 2 | sodium cacodylate |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | 250 (mM) | ||
| 10 | 1 | drop | 75 (mM) | ||
| 11 | 1 | drop | 200 (mM) | ||
| 12 | 1 | drop | MPD | 10 (%) | |
| 2 | 1 | reservoir | 75 (mM) | ||
| 3 | 1 | reservoir | 25 (mM) | ||
| 4 | 1 | reservoir | 2-mercaptoethanol | 6 (mM) | |
| 5 | 1 | reservoir | potassium cacodylate | 0.1 (M) | or 0.1M MES |
| 6 | 1 | reservoir | MPD | 13-17 (%) | |
| 7 | 1 | drop | protein | 10 (mg/ml) | |
| 8 | 1 | drop | Tris-HCl | 20 (mM) | |
| 9 | 1 | drop | 25 (mM) |
