1IBK
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2001-02-24 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.99187 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 401.160, 401.160, 176.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 316.230 - 3.310 |
R-factor | 0.229 * |
Rwork | 0.229 |
R-free | 0.28200 |
Structure solution method | DIFFERENCE FOURIER |
RMSD bond length | 0.007 |
RMSD bond angle | 28.400 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 316.230 | 3.430 |
High resolution limit [Å] | 3.310 | 3.310 |
Rmerge | 0.160 * | 0.664 * |
Total number of observations | 1079968 * | |
Number of reflections | 207340 | |
<I/σ(I)> | 10.8 | 2.7 |
Completeness [%] | 97.8 | 98 |
Redundancy | 5.21 | 4.41 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | Wimberly, B.T., (2000) Nature, 407, 327.,Trakhanov, S.D., (1987) FEBS Lett., 220, 319. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NH4Cl | ||
10 | 1 | 2 | MPD | ||
11 | 1 | 3 | PAROMOMYCIN | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | magnesium acetate | ||
4 | 1 | 1 | sodium cacodylate | ||
5 | 1 | 1 | MPD | ||
6 | 1 | 2 | NH4Cl | ||
7 | 1 | 2 | KCl | ||
8 | 1 | 2 | magnesium acetate | ||
9 | 1 | 2 | sodium cacodylate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 250 (mM) | ||
10 | 1 | drop | 75 (mM) | ||
11 | 1 | drop | 200 (mM) | ||
12 | 1 | drop | MPD | 10 (%) | |
2 | 1 | reservoir | 75 (mM) | ||
3 | 1 | reservoir | 25 (mM) | ||
4 | 1 | reservoir | 2-mercaptoethanol | 6 (mM) | |
5 | 1 | reservoir | potassium cacodylate | 0.1 (M) | or 0.1M MES |
6 | 1 | reservoir | MPD | 13-17 (%) | |
7 | 1 | drop | protein | 10 (mg/ml) | |
8 | 1 | drop | Tris-HCl | 20 (mM) | |
9 | 1 | drop | 25 (mM) |