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1IBK

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]90
Detector technologyCCD
Collection date2001-02-24
DetectorCUSTOM-MADE
Wavelength(s)0.99187
Spacegroup nameP 41 21 2
Unit cell lengths401.160, 401.160, 176.360
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution316.230 - 3.310
R-factor0.229

*

Rwork0.229
R-free0.28200
Structure solution methodDIFFERENCE FOURIER
RMSD bond length0.007
RMSD bond angle28.400

*

Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]316.2303.430
High resolution limit [Å]3.3103.310
Rmerge0.160

*

0.664

*

Total number of observations1079968

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Number of reflections207340
<I/σ(I)>10.82.7
Completeness [%]97.898
Redundancy5.214.41
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5

*

4

*

Wimberly, B.T., (2000) Nature, 407, 327.,Trakhanov, S.D., (1987) FEBS Lett., 220, 319.

*

Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111NH4Cl
1012MPD
1113PAROMOMYCIN
211KCl
311magnesium acetate
411sodium cacodylate
511MPD
612NH4Cl
712KCl
812magnesium acetate
912sodium cacodylate
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoir250 (mM)
101drop75 (mM)
111drop200 (mM)
121dropMPD10 (%)
21reservoir75 (mM)
31reservoir25 (mM)
41reservoir2-mercaptoethanol6 (mM)
51reservoirpotassium cacodylate0.1 (M)or 0.1M MES
61reservoirMPD13-17 (%)
71dropprotein10 (mg/ml)
81dropTris-HCl20 (mM)
91drop25 (mM)

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건을2024-03-27부터공개중

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