1I3C
RESPONSE REGULATOR FOR CYANOBACTERIAL PHYTOCHROME, RCP1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.410, 76.990, 43.040 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.900 |
| R-factor | 0.188 * |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.155 |
| Total number of observations | 83143 * | |
| Number of reflections | 20025 * | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293-295 * | Ammonium Sulfate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293-295 * | Ammonium Sulfate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293-295 * | Ammonium Sulfate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 2 | 1 | reservoir | ammonium sulfate | 3.1 (M) |
