1HYN

CRYSTAL STRUCTURE OF THE CYTOPLASMIC DOMAIN OF HUMAN ERYTHROCYTE BAND-3 PROTEIN

Experimental procedure

実験手法	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	108
Detector technology	CCD
Collection date	1998-09-23
Detector	ADSC QUANTUM 1
Wavelength(s)	0.9789, 0.9796, 0.9537
Spacegroup name	C 1 2 1
格子定数 [Å]	181.590, 92.580, 123.330
格子定数 [度]	90.00, 131.86, 90.00

精密化法

残基	8.000 - 2.600
R因子	0.216 *
Rwork	0.216
R-free	0.29000
Structure solution method	MAD
結合長の平均二乗偏差(RMSD) [Å]	0.008 *
結合角の平均二乗偏差(RMSD) [度]	1.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	35.000 *	2.680
分解能 [Å] (高)	2.590	2.590
Rmerge_l_obs	0.057 *	0.208 *
独立反射数	46609
<I/σ(I)>	37.16	18.7
完全性 [%]	99.1 *	94.5 *
冗長性	3.8	2.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, sitting drop *	7.4 *		Kiyatkin, A., (1995) Proteins: Struct., Funct., Genet., 22, 293. *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	1.2-2 (mg/ml)
10	1	reservoir	sodium azide	1 (mM)
2	1	drop	sodium phosphate	5 (mM)
3	1	drop		150 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	sodium azide	1 (mM)
6	1	drop	dithiothreitol	1 (mM)
7	1	drop	glycerol	30 (%(v/v))
8	1	reservoir	ammonium sulfate	65-68 (%sat)
9	1	reservoir	sodium citrate	150 (mM)