1HW2
FADR-DNA COMPLEX: TRANSCRIPTIONAL CONTROL OF FATTY ACID METABOLISM IN ECHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-02 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.733, 110.148, 130.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.250 |
| R-factor | 0.235 * |
| Rwork | 0.235 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native FadR dimer |
| RMSD bond length | 0.007 |
| RMSD bond angle | 19.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.072 | 0.346 |
| Total number of observations | 643902 * | |
| Number of reflections | 20310 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.2 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 18 * | isopropanol, MES, magnesium chloride, pH 6.0, EVAPORATION, temperature 290.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | isopropanol | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | MgCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | isopropyl alcohol | 10 (%) | |
| 3 | 1 | reservoir | MES | 50 (mM) | pH6.0 |
| 4 | 1 | reservoir | 10 (mM) |
