1HR0
CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 399.581, 399.581, 176.320 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 99.000 * - 3.200 |
| R因子 | 0.218 * |
| Rwork | 0.218 |
| R-free | 0.26090 |
| Structure solution method | Difference Fourier using 30S as starting model |
| Starting model (for MR) | 1FJF |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 28.450 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 29.800 | 3.310 |
| 分解能 [Å] (高) | 3.200 | 3.200 |
| Rmerge_l_obs | 0.140 | 0.499 |
| Total number of observations | 854384 * | |
| 独立反射数 | 227537 * | |
| <I/σ(I)> | 5.1 | 1.92 |
| 完全性 [%] | 96.2 | 92.2 |
| 冗長性 | 3.75 | 3.12 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | unknown * | 6.5 | 277 | Wimberly, B.T., (2000) Nature, 407, 327. * |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | NH4Cl | ||
| 10 | 1 | 2 | MPD | ||
| 11 | 1 | 3 | initiation factor 1 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | potasium cacodylate | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | NH4Cl | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | magnesium acetate | ||
| 9 | 1 | 2 | potasium cacodylate |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 4 | 1 | 2-mercaptoethanol | 6 (mM) | ||
| 5 | 1 | potassium cacodylate | 0.1 (M ) | or 0.1M MES | |
| 6 | 1 | 1 | MPD | 13-17 (%) |
