1HNZ
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH HYGROMYCIN B
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93950 |
Spacegroup name | P 41 21 2 |
格子定数 [Å] | 402.063, 402.063, 175.263 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 99.000 * - 3.300 |
R因子 | 0.218 |
Rwork | 0.218 |
R-free | 0.26100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1FJF |
結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
結合角の平均二乗偏差(RMSD) [度] | 28.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 99.000 | 3.420 |
分解能 [Å] (高) | 3.300 | 3.300 |
Rmerge_l_obs | 0.158 * | 0.385 * |
Total number of observations | 630088 * | |
独立反射数 | 201587 | |
<I/σ(I)> | 4.7 | 2.07 |
完全性 [%] | 93.0 | 83.7 |
冗長性 | 2.9 | 1.65 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | unknown * | 6.5 | 277 | Wimberly, B.T., (2000) Nature, 407, 327. * |
結晶化試薬
ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
1 | 1 | 1 | NH4Cl | ||
10 | 1 | 2 | magnesium acetate | ||
11 | 1 | 2 | sodium cacodylate | ||
12 | 1 | 2 | MPD | ||
13 | 1 | 3 | HYGROMYCIN B | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | magnesium acetate | ||
5 | 1 | 1 | sodium cacodylate | ||
6 | 1 | 1 | MPD | ||
7 | 1 | 2 | NH4Cl | ||
8 | 1 | 2 | KCl | ||
9 | 1 | 2 | CaCl2 |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
4 | 1 | 2-mercaptoethanol | 6 (mM) | ||
5 | 1 | potassium cacodylate | 0.1 (M ) | or 0.1M MES |