1HG4
Ultraspiracle ligand binding domain from Drosophila melanogaster
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 37.980, 86.190, 137.000 |
Unit cell angles | 85.57, 85.94, 83.09 |
Refinement procedure
Resolution | 34.000 - 2.400 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ere |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | |
High resolution limit [Å] | 2.200 | 2.400 * |
Rmerge | 0.126 | 0.305 * |
Total number of observations | 175324 * | |
Number of reflections | 60384 | |
<I/σ(I)> | 4.4 | |
Completeness [%] | 90.0 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 * | 18 * | used streak-seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 130 (mM) | |
3 | 1 | reservoir | sodium acetate | 160 (mM) | |
4 | 1 | reservoir | PEG2000 | 25 (%) |