1HCK
HUMAN CYCLIN-DEPENDENT KINASE 2
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 72.820, 72.660, 54.070 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 8.000 - 1.900 |
| R因子 | 0.185 |
| Rwork | 0.185 |
| R-free | 0.27200 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 24.580 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | |
| 分解能 [Å] (高) | 1.900 | 1.900 * |
| Rmerge_l_obs | 0.067 | |
| 独立反射数 | 22636 | 3019 * |
| 完全性 [%] | 97.3 | 92.8 * |
| 冗長性 | 3.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 7.4 * | 4 * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | HEPES | 200 (mM) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) |
