1H8V
The X-ray Crystal Structure of the Trichoderma reesei Family 12 Endoglucanase 3, Cel12A, at 1.9 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.720, 71.580, 121.400 |
| Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
| Resolution | 28.100 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.23300 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 | 0.220 |
| Total number of observations | 230270 * | |
| Number of reflections | 87132 | |
| <I/σ(I)> | 15.9 | 4 |
| Completeness [%] | 92.0 | 84.5 |
| Redundancy | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 20-24 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | cacodylate | 200 (mM) | |
| 3 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 4 | 1 | reservoir | mPEG2000 | 10-30 (%(w/w)) |
