1H0D
Crystal structure of Human Angiogenin in complex with Fab fragment of its monoclonal antibody mAb 26-2F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.580, 72.530, 86.990 |
Unit cell angles | 90.00, 112.46, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1FVC 3HFL 1tet 1b1i |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.061 | 0.200 |
Number of reflections | 46470 | |
<I/σ(I)> | 17.6 | 4.92 |
Completeness [%] | 98.9 | 97.8 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 * | 16 * | 30% PEG 3350 AND 0.2M LITHIUM SULPHATE PH 6.0-7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 30 (%) | |
2 | 1 | reservoir | 0.2 (M) | pH6.0-7.5 | |
3 | 1 | drop | protein | 20 (mg/ml) | pH7.2 |