1GSF
GLUTATHIONE TRANSFERASE A1-1 COMPLEXED WITH ETHACRYNIC ACID
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-21 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.800, 95.600, 105.300 |
| Unit cell angles | 90.00, 92.20, 90.00 |
Refinement procedure
| Resolution | 7.500 - 2.700 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 21.500 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.700 * |
| Rmerge | 0.078 |
| Total number of observations | 41606 * |
| Number of reflections | 26072 |
| Completeness [%] | 94.7 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 55 (mg/ml) | |
| 2 | 1 | drop | PEG2000 | 10-20 (%) | |
| 3 | 1 | drop | Tris-HCl | 100 (mM) | |
| 4 | 1 | drop | NaAc | 30 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 1 (%) | |
| 6 | 1 | reservoir | PEG2000 | 10-20 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | NaAc | 30 (mM) | |
| 9 | 1 | reservoir | beta-mercaptoethanol | 1 (%) |
