1GP7
Acidic Phospholipase A2 from venom of Ophiophagus Hannah
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.920, 62.940, 57.160 |
Unit cell angles | 90.00, 100.93, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.600 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1poa |
RMSD bond length | 0.006 |
RMSD bond angle | 22.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.087 | 0.205 |
Total number of observations | 32758 * | |
Number of reflections | 11965 * | |
<I/σ(I)> | 15 | 5 |
Completeness [%] | 93.8 | 75 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 291 * | Wang, Z., (2001) Acta Crystallogr.,Sect.D, D57, 709. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | PEG4000 | 5 (%) | |
3 | 1 | drop | ethylene glycol | 4 (%) | |
4 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
5 | 1 | reservoir | PEG4000 | 10 (%) | |
6 | 1 | reservoir | ethylene glycol | 8 (%) |