Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1GP7

Acidic Phospholipase A2 from venom of Ophiophagus Hannah

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Temperature [K]293
Detector technologyIMAGE PLATE
DetectorMARRESEARCH
Spacegroup nameC 1 2 1
Unit cell lengths117.920, 62.940, 57.160
Unit cell angles90.00, 100.93, 90.00
Refinement procedure
Resolution8.000 - 2.600
R-factor0.205
Rwork0.205
R-free0.23300
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1poa
RMSD bond length0.006
RMSD bond angle22.600

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.690
High resolution limit [Å]2.6002.600
Rmerge0.0870.205
Total number of observations32758

*

Number of reflections11965

*

<I/σ(I)>155
Completeness [%]93.875
Redundancy2.52.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7.5291

*

Wang, Z., (2001) Acta Crystallogr.,Sect.D, D57, 709.

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein5 (mg/ml)
21dropPEG40005 (%)
31dropethylene glycol4 (%)
41dropHEPES0.1 (M)pH7.5
51reservoirPEG400010 (%)
61reservoirethylene glycol8 (%)

217705

数据于2024-03-27公开中

PDB statisticsPDBj update infoContact PDBjnumon