1GIK
POKEWEED ANTIVIRAL PROTEIN FROM SEEDS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1994-04-22 |
Detector | FUJI |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 84.760, 78.050, 91.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.24200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1paf |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.698 * |
Data reduction software | CCP4 |
Data scaling software | WEIS |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.296 * |
Total number of observations | 68617 * | |
Number of reflections | 23204 * | |
Completeness [%] | 82.0 * | 69.5 * |
Redundancy | 3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.5 | 306 * | Li, H.M., (1998) ACTA CRYSTALLOGR.,SECT.D, 54, 137. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 100 (mg/ml) | |
2 | 1 | drop | 0.03 (N) | ||
3 | 1 | drop | PEG6000 | 30 (%(w/v)) | |
4 | 1 | drop | 0.2 (M) | pH5.0-6.0 | |
5 | 1 | reservoir | PEG6000 | 30 (%) |