1GIK
POKEWEED ANTIVIRAL PROTEIN FROM SEEDS
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1994-04-22 |
Detector | FUJI |
Spacegroup name | I 2 2 2 |
格子定数 [Å] | 84.760, 78.050, 91.570 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 20.000 * - 1.800 |
R因子 | 0.183 |
Rwork | 0.183 |
R-free | 0.24200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1paf |
結合長の平均二乗偏差(RMSD) [Å] | 0.010 * |
結合角の平均二乗偏差(RMSD) [度] | 1.698 * |
Data reduction software | CCP4 |
Data scaling software | WEIS |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 20.000 | 1.850 |
分解能 [Å] (高) | 1.800 | 1.800 |
Rmerge_l_obs | 0.062 | 0.296 * |
Total number of observations | 68617 * | |
独立反射数 | 23204 * | |
完全性 [%] | 82.0 * | 69.5 * |
冗長性 | 3 | 2 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion * | 5.5 | 306 * | Li, H.M., (1998) ACTA CRYSTALLOGR.,SECT.D, 54, 137. * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 100 (mg/ml) | |
2 | 1 | drop | 0.03 (N) | ||
3 | 1 | drop | PEG6000 | 30 (%(w/v)) | |
4 | 1 | drop | 0.2 (M) | pH5.0-6.0 | |
5 | 1 | reservoir | PEG6000 | 30 (%) |