1GHD
Crystal structure of the glutaryl-7-aminocephalosporanic acid acylase by mad phasing
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 115 |
| Detector technology | CCD |
| Collection date | 2000-11-08 |
| Detector | SBC-2 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.480, 73.480, 382.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| Rwork | 0.218 |
| R-free | 0.26500 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.290 |
| Data reduction software | DENZO (2000) |
| Data scaling software | SCALEPACK (2000) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.128 | 0.480 |
| Number of reflections | 68105 | |
| <I/σ(I)> | 30.5 | |
| Completeness [%] | 98.6 | 97.1 |
| Redundancy | 7.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 291 | Ichikawa, S., (1981) Agric. Biol. Chem., 45, 2231. * |
