1GGG
GLUTAMINE BINDING PROTEIN OPEN LIGAND-FREE STRUCTURE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1990-07-25 |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.300, 86.300, 81.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.28900 |
Structure solution method | ISIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | XENGEN (V. 2.0) |
Data scaling software | XENGEN (V. 2.0) |
Phasing software | ISIR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 2.460 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.051 | 0.251 |
Total number of observations | 100231 * | |
Number of reflections | 24247 | |
<I/σ(I)> | 17.37 | 2.045 |
Completeness [%] | 92.6 | 61 |
Redundancy | 4.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.6 | 18 * | pH 8.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 0.1 (M) | |
3 | 1 | drop | ammonium sulfate | 50 (%sat) | |
4 | 1 | reservoir | ammonium sulfate | 75 (%sat) |