1G6K
Crystal structure of glucose dehydrogenase mutant E96A complexed with NAD+
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-06-06 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 121.955, 66.640, 120.065 |
| 格子定数 [度] | 90.00, 93.44, 90.00 |
精密化法
| 残基 | 39.950 - 2.000 |
| Rwork | 0.219 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gco |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 100.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.076 | 0.208 |
| 独立反射数 | 294512 | |
| <I/σ(I)> | 17.7 | |
| 完全性 [%] | 94.6 | 80.2 |
| 冗長性 | 4.78 | 2.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG2000, sodium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
