1G17
CRYSTAL STRUCTURE OF SEC4-GUANOSINE-5'-(BETA,GAMMA)-IMIDOTRIPHOSPHATE
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9802,0.9800,0.9537 |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 84.504, 84.504, 86.469 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 33.700 - 2.000 |
| R因子 | 0.264 |
| Rwork | 0.264 |
| R-free | 0.29200 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 22.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 38.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.091 | 0.624 |
| Total number of observations | 87371 * | |
| 独立反射数 | 23496 | |
| <I/σ(I)> | 13.2 | |
| 完全性 [%] | 99.7 | 99.4 |
| 冗長性 | 3.72 | 3.48 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8 * | 21 * | drop consists of equal amounts of protein and reservoir solutions * |
| 1 | Vapor diffusion, hanging drop * | 8 * | 21 * | drop consists of equal amounts of protein and reservoir solutions * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | reservoir | PEG4000 | 25 (%(v/v)) | |
| 7 | 1 | reservoir | ethylene glycol | 25-30 (%(v/v)) | |
| 8 | 1 | reservoir | 0.2 (M) | ||
| 9 | 1 | reservoir | sodium HEPES | 100 (mM) |
