1FV9
Crystal structure of human microurokinase in complex with 2-amino-5-hydroxy-benzimidazole
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 160 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 55.160, 53.000, 82.300 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 10.000 - 3.000 |
| R因子 | 0.248 |
| Rwork | 0.248 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.026 |
| 結合角の平均二乗偏差(RMSD) [度] | 28.900 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (98.0) |
Quality characteristics
| Overall | |
| 分解能 [Å] (高) | 3.000 * |
| Rmerge_l_obs | 0.101 * |
| 完全性 [%] | 99.0 * |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 * | 18-24 * | 0.15M LiSo4, 20% PEG 4000, succinate buffer, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | citrate | 10 (mM) | |
| 3 | 1 | drop | epsilon-amino caproic acid rho-carbethoxyphenyl ester chloride | 3 (mM) | |
| 4 | 1 | drop | DMSO cosolvent | 1 (%) | |
| 5 | 1 | reservoir | 0.15 (M) | ||
| 6 | 1 | reservoir | PEG4000 | 20 (%) | |
| 7 | 1 | reservoir | succinate |
