1FTS
SIGNAL RECOGNITION PARTICLE RECEPTOR FROM E. COLI
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.559, 79.577, 59.206 |
Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
Resolution | 7.500 - 2.200 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.28000 |
Structure solution method | MIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.560 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.4) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.038 * |
Number of reflections | 15365 |
<I/σ(I)> | 15 |
Completeness [%] | 98.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 4 * | VAPOR DIFFUSION, pH 6.0, vapor diffusion |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | bis-Tris | 50 (mM) | |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | PEG8000 | 18 (%) |