1FQ1
CRYSTAL STRUCTURE OF KINASE ASSOCIATED PHOSPHATASE (KAP) IN COMPLEX WITH PHOSPHO-CDK2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-01 |
Detector | ADSC |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 134.450, 134.450, 65.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.235 * |
Rwork | 0.235 |
R-free | 0.31300 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.130 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.073 | 0.360 |
Total number of observations | 89741 * | |
Number of reflections | 17091 | |
<I/σ(I)> | 5.6 | |
Completeness [%] | 99.7 | 99.6 * |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 8000,KCl, Mgacetate,Na cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | AMPPNP | 5 (mg) | |
3 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
4 | 1 | reservoir | PEG8000 | 8-10 (%(w/v)) | |
5 | 1 | reservoir | 0.2 (M) | ||
6 | 1 | reservoir | magnesium acetate | 100 (mM) |