1F63
CRYSTAL STRUCTURE OF DEOXY SPERM WHALE MYOGLOBIN MUTANT Y(B10)Q(E7)R(E10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.520, 91.520, 45.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.22000 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | FFT ((POINT MUTANT)) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.095 | 0.249 |
| Number of reflections | 19613 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 94.2 | 89.3 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.7 | 21 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | drop | sodium dithionite | 10 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.7 (M) | |
| 4 | 1 | reservoir | Tris-Cl | 20 (mM) |
