1EVV
CRYSTAL STRUCTURE OF YEAST PHENYLALANINE TRANSFER RNA AT 2.0 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-04-20 |
Detector | ADSC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.252, 32.988, 61.882 |
Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
Resolution | 25.790 - 2.000 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26300 |
Starting model (for MR) | 1tra |
RMSD bond length | 0.009 |
RMSD bond angle | 12.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.800 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.036 * | 0.171 * |
Total number of observations | 30638 * | |
Number of reflections | 13678 | |
<I/σ(I)> | 14.8 | 2.2 |
Completeness [%] | 88.6 | 70.7 |
Redundancy | 3.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7.5 | 4 * | CRYSTALS GROWN AT 277 K IN 0.010 M TRIS-HCL PH 7.5, 0.012 M MGCL2, 0.002 M SPERMINE, 6% 1,6-HEXANEDIOL. CRYOPROTECTION CONDITIONS: AFTER WASHING IN FRESH CRYSTALLIZATION SOLUTION FOR 10 MIN AND ADDITION OF 30% MPD (V/V), CRYSTALS WERE FLASH-FROZEN IN LIQUID NITROGEN, DIALYSIS, temperature 277.15K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | TRIS-HCL | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | SPERMINE | ||
4 | 1 | 1 | 1,6-HEXANEDIOL | ||
5 | 1 | 2 | MGCL2 | ||
6 | 1 | 2 | 1,6-HEXANEDIOL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | tRNA | 4 (mg/ml) | ||
2 | 1 | 2 | Tris-HCl | 10 (mM) | |
3 | 1 | 2 | 12 (mM) | ||
4 | 1 | 2 | spermine | 2 (mM) | |
5 | 1 | 1,6-hexanediol | 6 (%(v/v)) |