1EO7
BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE IN COMPLEX WITH MALTOHEXAOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.648, 109.022, 64.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.480 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cxl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.719 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.000 | 2.550 |
| High resolution limit [Å] | 2.480 | 2.490 |
| Rmerge | 0.061 | 0.225 |
| Number of reflections | 26146 | |
| <I/σ(I)> | 14.36 | 4.2 |
| Completeness [%] | 91.2 | 86.4 |
| Redundancy | 3.27 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.1 * | 293 | Lawson, C.L., (1994) J. Mol. Biol., 236, 590. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 60 (%(v/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | maltose | 5 (%(w/v)) |
