1EJO
FAB FRAGMENT OF NEUTRALISING MONOCLONAL ANTIBODY 4C4 COMPLEXED WITH G-H LOOP FROM FMDV.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1998-08-12 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.180, 69.328, 146.539 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
Rwork | 0.248 |
R-free | 0.26240 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.360 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.093 | 0.240 |
Number of reflections | 24105 | |
<I/σ(I)> | 4.86 | |
Completeness [%] | 91.7 | 96.7 |
Redundancy | 96.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PEG 4K 16%, LiCl 0.2 M, Tris HCl 50mM , pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Fab | 7 (mg/ml) | |
2 | 1 | drop | peptide | 1.8 (mg/ml) | |
3 | 1 | drop | PEG4000 | 6.5 (%) | |
4 | 1 | drop | 0.2 (M) | ||
5 | 1 | drop | Tris-HCl | 50 (mM) | |
6 | 1 | reservoir | PEG4000 | 13 (%) |