1EGE
STRUCTURE OF T255E, E376G MUTANT OF HUMAN MEDIUM CHAIN ACYL-COA DEHYDROGENASE
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1993-06-21 |
Detector | RIGAKU |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 169.590, 169.590, 151.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.750 |
R-factor | 0.221 |
Rwork | 0.221 |
RMSD bond length | 0.009 |
RMSD bond angle | 22.300 * |
Data reduction software | R-AXIS (IIC) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.750 * |
Rmerge | 0.107 * |
Total number of observations | 104904 * |
Number of reflections | 44572 |
Completeness [%] | 79.0 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 0.027mg | |
2 | 1 | drop | Tris acetate | 140 (mM) | |
3 | 1 | drop | PEG8000 | 8.0 (%(w/v)) | |
4 | 1 | reservoir | PEG4000 | 50 (%) |