1EEU
M4L/Y(27D)D/Q89D/T94H mutant of LEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-06 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.900, 104.800, 42.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.195 * |
| Rwork | 0.194 |
| R-free | 0.22200 |
| Structure solution method | Rigid body fitting |
| Starting model (for MR) | 1EEQ WITH ATOMS BEYOND CB REMOVED FOR RESIDUES A 89 AND B 389 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | 0.263 * |
| Number of reflections | 37705 | |
| <I/σ(I)> | 43.6 | 7.3 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 18 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20% PEG4K, 20% 2-Propanol, 0.1 M Sodium Citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | 2-propanol | 20 (%) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 4 | 1 | drop | protein | 10 (mg/ml) |
