1E96

Structure of the Rac/p67phox complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-15
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
格子定数 [Å]	83.220, 83.220, 138.530
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	15.000 - 2.400
R因子	0.248
Rwork	0.244
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mh1 1a17
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.390
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	15.000	2.450
分解能 [Å] (高)	2.400	2.400
Rmerge_l_obs	0.084	0.450
Total number of observations	123873 *
独立反射数	20975 *
<I/σ(I)>	16.9	4.2
完全性 [%]	94.0	100
冗長性	5.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	7.5 *	18 *	0.1 M CAPS PH 10.5, 0.2 M LI ACETATE, 0.8 M NAH2PO4, 1.2 M K2HPO4

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop		5 (mM)
6	1	reservoir	Caps	0.1 (M)
7	1	reservoir	lithium acetate	0.2 (M)
8	1	reservoir	sodium phophate	0.8 (M)
9	1	reservoir	potassium phosphate	1.2 (M)