1E2O
CATALYTIC DOMAIN FROM DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1994-03 |
Detector | MARRESEARCH |
Spacegroup name | F 4 3 2 |
格子定数 [Å] | 222.800, 222.800, 222.800 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 20.000 - 3.000 |
R因子 | 0.205 |
Rwork | 0.205 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eaa |
結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
結合角の平均二乗偏差(RMSD) [度] | 24.700 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((ROTAVATA) |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 20.000 | 3.160 |
分解能 [Å] (高) | 3.000 | 3.000 |
Rmerge_l_obs | 0.046 * | 0.145 * |
独立反射数 | 9906 | |
<I/σ(I)> | 13 | 4.9 |
完全性 [%] | 98.5 | 100 |
冗長性 | 4.1 | 4.5 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 7.3 * | PROTEIN WAS CRYSTALLIZED FROM 1.2M AMMONIUM SULFATE, 1% ETHANOL, 50 MM POTASSIUM PHOSPHATE, PH 7.0 |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 50 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | benzamidine | 1 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
7 | 1 | reservoir | ethanol | 1 (%(v/v)) |