1DWW
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-hydroxyarginine and dihydrobiopterin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 212.980, 212.980, 114.200 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 30.000 - 2.350 |
| R因子 | 0.249 |
| Rwork | 0.249 |
| R-free | 0.28800 |
| Structure solution method | OTHER |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 23.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.4) |
| Refinement software | X-PLOR |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.430 |
| 分解能 [Å] (高) | 2.350 | 2.350 |
| Rmerge_l_obs | 0.074 * | 0.289 * |
| Total number of observations | 303449 * | |
| 独立反射数 | 62465 | |
| <I/σ(I)> | 28.9 | 7.4 |
| 完全性 [%] | 98.7 | 96.7 * |
| 冗長性 | 4.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 7.6 * | 4 * | pH 6.00 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 10 | 1 | reservoir | 18-21 (%) | or ammonium sulfate | |
| 2 | 1 | drop | HEPPS | 40 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | NHA | 4 (mM) | |
| 6 | 1 | drop | 2 (mM) | ||
| 7 | 1 | drop | 4-amino-H4B | 2 (mM) | |
| 8 | 1 | reservoir | MES | 50 (mM) | |
| 9 | 1 | reservoir | beta-octylglucoside | 50 (mM) |
