1DUX

ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-02-15
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
格子定数 [Å]	33.245, 140.523, 38.620
格子定数 [度]	90.00, 115.52, 90.00

精密化法

残基	50.000 - 2.100
R因子	0.202 *
Rwork	0.202
R-free	0.23600
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	1.089
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.180
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.042	0.092
独立反射数	17831
<I/σ(I)>	27.1
完全性 [%]	95.8	77.6
冗長性	3.9	2.01

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	5.6	20 *	50 mM sodium Cacodylate, 10% PEG 2000, 100 mM MgCl2, 100 mM NaCl, 3 mM ZnCl2, pH 5.6, VAPOR DIFFUSION, temperature 293K
1	Vapor diffusion, hanging drop *	5.6	20 *	50 mM sodium Cacodylate, 10% PEG 2000, 100 mM MgCl2, 100 mM NaCl, 3 mM ZnCl2, pH 5.6, VAPOR DIFFUSION, temperature 293K

結晶化試薬

ID	結晶ID	濃度	試薬名	濃度 (単位)	詳細
1	1	1	sodium Cacodylate
2	1	1	MgCl2
3	1	1	NaCl
4	1	1	ZnCl2
5	1	1	PEG 2000
6	1	2	PEG 2000

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	0.1 (mM)
10	1	reservoir		100 (mM)
11	1	reservoir		100 (mM)
12	1	reservoir		3.0 (mM)
2	1	drop	DNA	0.13 (mM)
3	1	drop	sodium cacodylate	25 (mM)
4	1	drop	PEG2000	5 (%(w/v))
5	1	drop		50 (mM)
6	1	drop		50 (mM)
7	1	drop		1.5 (mM)
8	1	reservoir	sodium cacodylate	50 (mM)
9	1	reservoir	PEG2000	10 (%(w/v))