1DM2
HUMAN CYCLIN-DEPENDENT KINASE 2 COMPLEXED WITH THE INHIBITOR HYMENIALDISINE
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-05 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 53.390, 70.670, 72.260 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 30.000 - 2.100 |
| R因子 | 0.192 |
| Rwork | 0.192 |
| R-free | 0.26700 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 25.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.160 |
| 分解能 [Å] (高) | 2.100 | 2.100 |
| Rmerge_l_obs | 0.048 | 0.223 |
| 独立反射数 | 16264 | |
| <I/σ(I)> | 21.2 | |
| 完全性 [%] | 98.1 | 86.6 |
| 冗長性 | 3.2 | 2.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 7.4 | 4 * | Rosenblatt, J., (1993) J. Mol. Biol., 230, 1317. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | HEPES | 200 (mM) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) |
