1DLF
HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 5.25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 112 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-08 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.101, 70.722, 53.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.450 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.23000 |
Structure solution method | MULTIPLE MOLECULAR REPLACEMENT |
Starting model (for MR) | FV PORTION OF FAB' B13I2 (1IGF) |
RMSD bond length | 0.008 |
RMSD bond angle | 28.290 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.067 | 0.265 |
Total number of observations | 280945 * | |
Number of reflections | 36158 | |
<I/σ(I)> | 35.3 | 8.3 |
Completeness [%] | 93.9 | 94.4 |
Redundancy | 8.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.25 | 293 * | pH 5.25 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 0.8 (M) | |
3 | 1 | drop | sodium cacodylate | 25 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
5 | 1 | reservoir | sodium cacodylate | 25 (mM) |