1DKY

THE SUBSTRATE BINDING DOMAIN OF DNAK IN COMPLEX WITH A SUBSTRATE PEPTIDE, DETERMINED FROM TYPE 2 NATIVE CRYSTALS

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	96.400, 117.000, 36.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.225
Rwork	0.225
R-free	0.30900
RMSD bond length	0.010
RMSD bond angle	25.250 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800	2.800 *
Rmerge	0.043 *	0.116 *
Total number of observations	23005 *
Number of reflections	9117
Completeness [%]	85.2	61.6 *
Redundancy	37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		or dialysis *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.4 (M)
2	1	reservoir	phosphate