1DAN
Complex of active site inhibited human blood coagulation factor VIIA with human recombinant soluble tissue factor
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.650, 82.550, 126.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.21800 |
RMSD bond length | 0.007 |
RMSD bond angle | 24.260 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.950 |
Rmerge | 0.076 |
Total number of observations | 162150 * |
Number of reflections | 54736 |
<I/σ(I)> | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sparse matrix screen * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Sephacryl S-200 column | 10 (mg/ml) |