1D9F
CRYSTAL STRUCTURE OF THE COMPLEX OF DNA POLYMERASE I KLENOW FRAGMENT WITH DNA TETRAMER CARRYING 2'-O-(3-AMINOPROPYL)-RNA MODIFICATION 5'-D(TT)-AP(U)-D(T)-3'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 1998-10-30 |
Detector | MARRESEARCH |
Spacegroup name | P 43 |
Unit cell lengths | 102.650, 102.650, 86.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.24300 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.630 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.000 |
High resolution limit [Å] | 3.000 * |
Rmerge | 0.078 |
Number of reflections | 20110 |
Completeness [%] | 99.9 |
Redundancy | 4.23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * | 295 | SODIUM CITRATE, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CITRATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mM) | |
2 | 1 | drop | potassium cacodylate | 40 (mM) | |
3 | 1 | drop | 80 (mM) | ||
4 | 1 | drop | spermine | 12 (mM) | |
5 | 1 | drop | MPD | 10 (%(v/v)) | |
6 | 1 | reservoir | MPD | 35 (%) |