1D8U

CRYSTAL STRUCTURE OF NON-SYMBIOTIC PLANT HEMOGLOBIN FROM RICE

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-08-21
Detector	FUJI
Spacegroup name	P 31 2 1
格子定数 [Å]	126.800, 126.800, 55.500
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	50.000 - 2.350
R因子	0.208 *
Rwork	0.208
R-free	0.26100
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	2.110
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.420
分解能 [Å] (高)	2.200	2.300 *
Rmerge_l_obs	0.690 *	0.223 *
独立反射数	21422 *	1634 *
<I/σ(I)>	13.7
完全性 [%]	80.0 *	46 *
冗長性	8.06	3.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7 *	295	AMMONIUM PHOSPHATE, SUCROSE, POTASSIUM PHOSPHATE, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 22K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	ammonium phosphate	2.2 (M)
2	1	reservoir	sucrose	20 (%)
3	1	drop	potassium phosphate	10 (mM)
4	1	drop	protein	2.2 (mM)