1D1W
BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN COMPLEXED WITH 2-AMINOTHIAZOLINE (H4B BOUND)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-13 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.460, 106.320, 156.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.25000 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | 0.700 |
Total number of observations | 174141 * | |
Number of reflections | 61326 | |
<I/σ(I)> | 9.3 | |
Completeness [%] | 89.7 | 84.4 |
Redundancy | 2.8 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 280 | PEG 4000, CACODYLATE, MAGNESIUM ACETATE THE CHEMICAL, S-AMINOETHYL-ISOTHIOUREA, ADDED TO THE CRYSTALLIZATION BUFFER UNDERGOES A SPONTANEOUS REARRANGEMENT TO FORM 2-AMINOTHIAZOLINE., pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 15 (%) | |
2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
3 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
4 | 1 | reservoir | SEITU | 1 (mM) | |
5 | 1 | reservoir | SDS | 0.035 (mM) | |
6 | 1 | reservoir | TCEP | 5 (mM) | or 5mM glutathione sulfonate |