1C8J

CRYSTAL STRUCTURE OF CYTOCHROME P450CAM MUTANT (F87W/Y96F)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	IMAGE PLATE
Collection date	2000-03-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
格子定数 [Å]	66.860, 61.990, 95.130
格子定数 [度]	90.00, 90.54, 90.00

精密化法

残基	100.000 - 2.100
R因子	0.193
Rwork	0.193
R-free	0.26400
結合長の平均二乗偏差(RMSD) [Å]	0.016
結合角の平均二乗偏差(RMSD) [度]	1.719
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	100.000	2.200
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.085	0.431
独立反射数	43708
<I/σ(I)>	7.4
完全性 [%]	95.6	98.1
冗長性	2.7	2.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 8000, Cacodylate, Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K