1C78

STAPHYLOKINASE (SAK) DIMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-11-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	43.870, 59.260, 102.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.204
Rwork	0.198
R-free	0.26000 *
RMSD bond length	0.020 *
RMSD bond angle	2.070 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.058 *	0.204 *
Total number of observations	48705 *
Number of reflections	12387 *
<I/σ(I)>	10.6
Completeness [%]	99.8 *	99.9 *
Redundancy	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	PEG1000	45-50 (%(w/v))
4	1	reservoir	Tris-HCl	100 (mM)