1BR3
CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.450, 63.450, 216.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.25100 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 28.300 * |
| Data reduction software | SOFTWARE (AT SYNCHROTRON) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.043 * | |
| Number of reflections | 5739 | |
| <I/σ(I)> | 5.8 | 3.8 |
| Completeness [%] | 98.2 | 98 |
| Redundancy | 4.1 | 4.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 24.5 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.4 (mM) | |
| 2 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 3 | 1 | reservoir | 30 (mM) | ||
| 4 | 1 | reservoir | spermine | 1 (mM) | |
| 5 | 1 | reservoir | 0.9 (M) |
