1BPX
DNA POLYMERASE BETA/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.767, 79.395, 54.622 |
| Unit cell angles | 90.00, 106.35, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.253 * |
| Rwork | 0.253 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpy |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MERLOT |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.047 * | |
| Total number of observations | 48472 * | |
| Number of reflections | 16536 | |
| Completeness [%] | 97.0 | 96.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | DNA | 0.005ml | |
| 2 | 1 | 2 | PEG3350 | 13.8 (%) | |
| 3 | 1 | 2 | sodium acetate | 360 (mM) | |
| 4 | 1 | 2 | imidazol | 50 (mM) |
