1BOB
HISTONE ACETYLTRANSFERASE HAT1 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH ACETYL COENZYME A
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-02 |
Detector | BRANDEIS |
Wavelength(s) | 0.93, 0.9789 |
Spacegroup name | C 1 2 1 |
格子定数 [Å] | 111.300, 47.900, 75.700 |
格子定数 [度] | 90.00, 90.30, 90.00 |
精密化法
残基 | 28.900 - 2.300 |
R因子 | 0.204 |
Rwork | 0.204 |
R-free | 0.26900 |
Structure solution method | MAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
結合角の平均二乗偏差(RMSD) [度] | 23.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | X-PLOR (3.843) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 28.900 | 2.340 |
分解能 [Å] (高) | 2.300 | 2.300 |
Rmerge_l_obs | 0.034 * | 0.139 * |
Total number of observations | 193352 * | |
独立反射数 | 18259 * | |
<I/σ(I)> | 20 | 5.29 |
完全性 [%] | 97.5 | 88.3 |
冗長性 | 4 | 4 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 7.6 | 4 * | pH 7.6 |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 2-fold molar excess | ||
3 | 1 | drop | HEPES | 2.5 (mM) | |
4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
5 | 1 | drop | 0.1 (M) | ||
6 | 1 | reservoir | PEG400 | 12 (%(w/v)) | |
7 | 1 | reservoir | Tris-Cl | 0.1 (M) |