1BM3
IMMUNOGLOBULIN OPG2 FAB-PEPTIDE COMPLEX
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-03-12 |
| Detector | SDMS |
| Spacegroup name | A 1 2 1 |
| 格子定数 [Å] | 74.110, 90.050, 79.110 |
| 格子定数 [度] | 90.00, 115.30, 90.00 |
精密化法
| 残基 | 8.000 - 2.000 |
| Rwork | 0.152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1opg |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.062 |
| Data reduction software | NILSEN |
| Data scaling software | X-GEN ((HOWARD) |
| Refinement software | X-PLOR (3.851) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 20.000 |
| 分解能 [Å] (高) | 2.000 |
| Rmerge_l_obs | 0.044 |
| 独立反射数 | 20334 |
| 完全性 [%] | 78.0 |
| 冗長性 | 2.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 22 * | 0.1M CACODYLATE, PH6.5, 0.2M CALCIUM ACETATE AND 16% PEG 8000 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein/peptide | 21 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 16 (%) | |
| 3 | 1 | reservoir | cacodylate | 0.1 (M) | |
| 4 | 1 | reservoir | calcium acetate | 0.2 (M) |
