1BCC
CYTOCHROME BC1 COMPLEX FROM CHICKEN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 169.590, 182.518, 240.573 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 12.000 - 3.160 |
| R因子 | 0.27 |
| Rwork | 0.270 |
| R-free | 0.31000 |
| Structure solution method | MIR AND NCS AVERAGING |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 * |
| 結合角の平均二乗偏差(RMSD) [度] | 1.613 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 3.270 | |
| 分解能 [Å] (高) | 3.160 | 3.160 |
| Rmerge_l_obs | 0.095 | 0.324 |
| Total number of observations | 556456 * | |
| 独立反射数 | 123869 | |
| <I/σ(I)> | 12.5 | 2.7 |
| 完全性 [%] | 91.6 | 84.5 |
| 冗長性 | 4.5 | 3.32 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 6.7 | 20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | KMES | 60 (mM) | pH6.7 |
| 2 | 1 | drop | PEG4000 | 8 (%) | |
| 3 | 1 | drop | K-MOPS 7.5 | 20 (mM) | |
| 4 | 1 | drop | n-octyl-beta-D-glucopyranoside | 20 (g/l) | |
| 5 | 1 | drop | 87.5 (mM) | ||
| 6 | 1 | drop | glycerol | 10 (%) | |
| 7 | 1 | reservoir | glycerol | 30 (%) |
