1B9E
HUMAN INSULIN MUTANT SERB9GLU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.560, 46.390, 51.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.500 - 2.500 |
| R-factor | 0.165 * |
| Rwork | 0.165 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ZN INSULIN DIMER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.480 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.300 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 0.389 |
| Total number of observations | 13549 * | |
| Number of reflections | 3538 | |
| <I/σ(I)> | 15.98 | 3.07 |
| Completeness [%] | 88.4 | 87.8 * |
| Redundancy | 3.83 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 3.8 * | HANGING DROP, 0.1M AMMONIA CITRATE, 0.12% CHROMIUM DICHLORIDE (W/V), 10% ACETONE (V/V), 4% DIMETHYL FORMAMIDE (V/V), PH 3.8, pH 3.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 0.03 (M) | ||
| 3 | 1 | reservoir | ammonium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | 0.12 (%) | ||
| 5 | 1 | reservoir | acetone | 10 (%(v/v)) | |
| 6 | 1 | reservoir | dimethylformamide | 4 (%) |
