1B38
HUMAN CYCLIN-DEPENDENT KINASE 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-21 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.333, 71.122, 72.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.180 |
| R-free | 0.25000 |
| Structure solution method | OTHER |
| Starting model (for MR) | UNPUBLISHED |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.042 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.390 |
| Total number of observations | 70729 * | |
| Number of reflections | 18076 | |
| <I/σ(I)> | 11 | 2.26 |
| Completeness [%] | 95.2 | 96.8 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | PROTEIN AT 10 MG/ML IN 10MM HEPES/HCL PH7.4, 15MM NACL WELL BUFFER CONTAINING 50MM AMMONIUM ACETATE, 12% PEG 3350, 100MM HEPES/HCL PH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 15 (mM) | ||
| 3 | 1 | drop | HEPES | 10 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 10-15 (%) | |
| 5 | 1 | reservoir | ammonium acetate | 50 (mM) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
