1B0Z
The crystal structure of phosphoglucose isomerase-an enzyme with autocrine motility factor activity in tumor cells
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-25 |
Detector | RIGAKU RAXIS II |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 75.100, 93.730, 171.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
Rwork | 0.185 |
R-free | 0.25800 |
Structure solution method | MIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.700 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.360 |
Number of reflections | 24024 | |
<I/σ(I)> | 12 | 3.2 |
Completeness [%] | 93.9 | 83.4 |
Redundancy | 4.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7 | CRYSTALS WERE GROWN BY HANGING DROP VAPOR DIFFUSION FROM 4-UL DROPLETS OF PROTEIN SOLUTION (15 MG/ML) IN 50 MM PHOSPHATE BUFFER (PH7.0) AND 0.2 M AMMONIUM PHOSPHATE AGAINST A RESERVOIR OF THE ABOVE BUFFER CONTAINING 0.4M AMMONIUM PHOSPHATE., VAPOR DIFFUSION, HANGING DROP |