1B01
TRANSCRIPTIONAL REPRESSOR COPG/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 40.300, 40.300, 221.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.560 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.31600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.450 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 * | 1.690 |
| High resolution limit [Å] | 2.560 * | 2.560 * |
| Rmerge | 0.062 * | 0.455 * |
| Total number of observations | 78954 * | |
| Number of reflections | 6252 * | |
| <I/σ(I)> | 10.3 | 3 |
| Completeness [%] | 96.3 * | 86.8 * |
| Redundancy | 5.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | MPD, HEPES, pH 6.70, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 2 | MPD | ||
| 4 | 1 | 2 | HEPES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 60-78 (%) | ||
| 2 | 1 | reservoir | 0.1 (M) |
